Chromatography has long been an
useful and important asset to chemistry and it operates on the simple principle
of color, be it light, heat or chemical reactions the methods may differ but
the basic concept remains the same. Chromatography is usually two types; one
involves analytical procedure wherein you just analyze the mixture and record
the contents, the other is separation procedure where the mixture of elements
is separated from a compound. Chromatography process has become lightening fast
unlike the olden methods which used to take days and even months to finish.
High
pressure liquid chromatograph otherwise called as High
Performance Liquid Chromatograph is being widely used by various
applications and the purpose of this chromatography technique is to separate
the mixtures. The process involves a mobile phase with which the sample is
mixed and sent in to a tube packed with several types of stationary phases
which are usually adsorbents.
Variety of HPLC techniques are available,
the basic and well known techniques is paper chromatography which is a type of
partition chromatography. In this type, the sample is introduced as a droplet
in the centre of the paper; note that sample is mixed with a mobile phase
before it is placed.
High performance liquid chromatography is named so since this technique has
provided good results and is much more faster than other normal chromatography
techniques; mainly due to the reason that it is forced and not natural but yet
never fails to deliver what exactly is needed.